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. 2016 Sep 30;6:34345. doi: 10.1038/srep34345

Figure 7. Early postnatal metformin treatment ameliorates thermogenic impairment in BAT due to maternal HFD feeding during lactation.

Figure 7

(a) Body weight of Con-saline, HFD-saline and HFD-metformin at PND21 and one-month of age (Con-saline: Con offspring injected with saline; HFD-saline: HFD offspring injected with saline; HFD-metformin: HFD offspring injected with metformin from PND2-PND21, then weaned on chow diet at PND21. n = 7–11 each group). (b) BAT serotonin content of Con-saline, HFD-saline and HFD-metformin mice at one month of age (n = 6 each group). (c) Coupled and uncoupled respiration rate of isolated BAT from Con-saline, HFD-saline and HFD-metformin mice at one-month of age (n = 4 per group). (d,e) UCP1 mRNA (d) and protein contents (e) in BAT of Con-saline, HFD-saline and HFD-metformin at one-month of age under cold-stimulus for 6 h (n = 6 per group). (f) Rectal temperature changes of Con-saline, HFD-saline and HFD-metformin mice at one-month of age during 6 h under cold exposure (n = 6–8 per group). Data are presented as Means ± S.E.M. *P < 0.05; **P < 0.01; ***P < 0.001 and ***P < 0.0001 were determined by One-way ANOVA with Tukey’s post doc test.