Figure 6. Adhesive ligand tether density does not affect the size and length of focal adhesions.

(a) Confocal microscopy images of cells stained for paxillin (green) and F-actin (red). Cells were seeded on short linkers diluted with non-reactive short linkers in a ratio 1:3 and 1:10. Scale bars are 20 μm in main image and cell inset and 5 μm in FAs inset. (b,c) Quantification of focal adhesion area and length (n > 100 for each condition). Histograms represent mean ± sem.