Table 1. Ectopic expression of Elba2 or BigH1 partially restores viability in H1-depleted animals.
| Cross | Act5C-GAL4/SM5 | Act5C-GAL4, pINT-H14M/SM5 | p-value |
|---|---|---|---|
| w1118 (wt control) | 119/256 (128), 93% | 24/187 (94), 26% | N/A |
| P{EP}Elba2G17999 | 125/246 (123), 102% | 69/193 (97), 72% | 2.1 · 10-7 |
| UAS-Elba2 (II) | 153/295 (148), 104% | 84/232 (116), 72% | 5.4 · 10-8 |
| UAS-hBEND6 (II) | 82/174 (87), 94% | 33/195 (98), 34% | 0.26 |
| UAS-insv (II) | 142/346 (173), 82% | 2/234 (117), 2% | 3.9 · 10-7 |
| P{EP}BigH1G18579 | 93/219 (110), 102% | 53/170 (102%), 102% | 2.6 · 10-5 |
All crosses were performed at 27 °C. Males homozygous for UAS mis-expression EP alleles or UAS-driven transgenes on the II chromosome were mated to heterozygous Act5C-GAL4/SM5, Cy or Act5C-GAL4, pINT-1-H14M/SM5, Cy females. Viability was scored as the number of eclosed Cy+ adults relative to the total number of offspring scored (columns 2 and 3). The expected numbers of Cy+ flies (calculated from the Mendelian distribution) are shown in parentheses; percent viability relative to the expected numbers is also shown. Low percentage numbers that indicate increased lethality are highlighted in red. Probability values are calculated by the chi-square two-way test (column 4). Statistically significant results (p < 0.05) are highlighted in bold typeface. N/A, not applicable.