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. 2016 Sep 30;6:34437. doi: 10.1038/srep34437

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Two independent mutant alleles at the rsph9 locus were obtained using CRISPR/Cas9 mutagenesis. (B,C): rsph9²⁰⁸/+ (B) and rsph9²⁰⁸/rsph9²⁰⁸ (C) siblings were fixed at 5 dpf and stained for acetylated α-tubulin (yellow) and Rsph9 (magenta). (D) Chart summarizing olfactory ciliary motility shows reduced motility in homozygous mutants.