Figure 4.

Free energy barrier heights of nucleosome unwrapping for 601 positioning sequence. Solid lines represent the unwrapping (forward) reactions, dotted lines represent wrapping (reverse) reactions. When the unwrapping and wrapping barriers are equal, the two basins are at equilibrium with one another. This is found when τ₁^* = 3.3 pN for the outer wrap, and τ₂ = 8.5 pN for the inner wrap. ΔA^†(τ₁^*) = 4 kT and ΔA^†(τ₂) = 16 kT. Error bars represent standard deviation across four independent simulations.