Figure 6.

In vivo delivery of DOX using CPK and CPE. 2 dpf zebrafish were treated with CPK for 30 min, followed by 30 min incubation with CPE-decorated liposomes (0.25 mM total lipid concentration and 1 mol % CPE) loaded with DOX. (A, B) Whole-embryo imaging showing widespread DOX delivery in living zebrafish embryos (control experiments in Supplementary Figure S9). (C–E) Single zebrafish skin epithelial cell (from the indicated site of the embryo in (A, B) displaying membrane associated DOPE-NBD labeling (NBD) and predominantly nuclear DOX labeling.