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. 2016 Aug 25;2(9):637–646. doi: 10.1021/acscentsci.6b00180

Figure 2.

Figure 2

Ammonium sulfate accelerated π-clamp mediated protein labeling. Shown are the structures (left), the yield-time curves for the protein labeling reaction (middle), and the deconvoluted mass spectra from LC−MS analysis of the crude reaction mixture (right) for pGB1 variants 3a (a) and 3b (b) reacting with perfluoroaryl probe 2 with and without 2 M ammonium sulfate. Reaction conditions: 0.1 mM protein 3a or 3b, 1 mM probe 2, 200 mM phosphate, 20 mM TCEP, with or without 2 M ammonium sulfate, 37 °C.