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Salt effect enabled site-selective cysteine alkylation. The competing alkylation reaction between the π-clamp peptide 1A and the double glycine mutant 1N using a hydrophobic alkylating probe 7. Reaction conditions: 0.1 mM 1A, 0.1 mM 1N, 1 mM DTT, 0.5 mM probe 7, 100 mM phosphate, 0.25% DMSO, pH 8.0, with or without 2 M ammonium sulfate, room temperature, 15 min.
