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. 2016 Sep 29;9:102. doi: 10.1186/s13045-016-0326-6

Fig. 3.

Fig. 3

Expression pattern of the hematopoietic progenitor markers CD43 and CD45 during iPSC commitment to CD34+ hematopoietic cells in 3D induction systems. Kinetic expression of CD43+ cells (ae) and CD45+ cells (fj) during the hematopoietic cell differentiation of iPSCs in different 3D induction systems fused the stromal cells and factors with flow cytometry analysis. On the initial induction day, cultured iPSCs expressed few CD43 (day 0) or CD45 (day 0). After culture in 3D induction systems for 5 days, cells expressed about 30 % percentage of CD43+ but less than 5 % of CD45+ cells. When the induction lasted to day 10, about 70 % of the cells expressed CD43; however, the level of CD45 expression remains low about 10 %