Fig. 5.

Hematopoietic colony-forming unit potential assay of CD34⁺ cells obtained from 3D induction systems. a Distribution of total hematopoietic colonies and colony subtypes obtained under differentiation of iPSCs in various 3D induction systems (error bars represented mean ± SEM; n = 3). Compared to no feeder control group, CFU potential of iPSC differentiating on 3D induction systems seeded with bone marrow and OP9DL1 held greater hematopoietic capacity. b Calculation of hematopoietic colony subtypes obtained under differentiation of iPSCs on the stromal cells derived from bone marrow and OP9DL1, indicating hematopoietic lineage forming from CD34⁺ hematopoietic cells (mean ± SEM; n = 3). c–n Various types of CFUs were observed as CFU-E (c), BFU-E (e), CFU-G (g), CFU-GM (i), CFU-ME (k), and CFU-GEMM (m) when CD34⁺ cells were cultured into CFU system, indicating the multi-potential differentiation capabilities of CD34+ cells derived from 3D induction systems. Morphologies of the hematopoietic lineages derived from CFUs were identified with Wright-Giemsa staining as shown in d, f, h, j, l, and n, respectively