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. 2016 Sep 29;9:102. doi: 10.1186/s13045-016-0326-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Production of mature hematopoietic cells in the following 3D culture systems. (Aa–Dc) The morphology was captured during the culture for 21–45 days. Some colonies were formed similar to CFUs in Methocult 4434 (A, B, and C). The relevant bright, immunofluorescent and overlapped field visions were shown in a, b, and c, respectively. Some cells were similar to the subtypes of lymphoid cells in morphology as shown in Da–Dc. E–H Kinetic expression of CD3⁺, CD19⁺, CD45⁺, and CD34⁺ cells to analyze the multi-lineage potential of CD34⁺ hematopoietic stem cells derived from iPSCs in vitro with flow cytometry assay. I Statistics analysis of CD3⁺, CD19⁺, CD45⁺, and CD34⁺ cells in 3D later induction system. Error bars represented mean ± SEM, n = 3