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. 2016 Sep 29;14:284. doi: 10.1186/s12967-016-1030-6

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Representative images of serial PAD gastrocnemius muscle sections stained for mitochondrial activity and fiber type. A succinate dehydrogenase (SDH) activity; B myosin heavy chain (MyHC) composition by immunohistochemistry; C cytochrome c oxidase activity. Arrows point to the same fiber in serial sections. Arrow, type I fiber (pink); double arrow, type IIa fiber (green); large arrowhead, type IIa/x hybrid fiber (yellow/orange). D Correlation between the percent of total fibers with SDH cavities and type I fiber frequency in PAD subjects (n = 18, approximately 1000 fibers counted per subject). Scale bar = 100 µm