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. 2016 Sep 29;18:97. doi: 10.1186/s13058-016-0757-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Low galectin-3 (Gal3) correlates with heightened tumorigenicity. a GI-LM2C (red) and GI-LM2G (green) sphere cells were injected into the fourth mammary fat pad of mice with rapid tumor growth in GI-LM2G (upper panel) (n ≥ 3). A representative picture of mice used in this study is shown (red arrow = injection site for GI-LM2C; green arrow = injection site for GI-LM2G). b In vivo tumorigenicity assay with limited dilution; shown are the tumor-free survival curves for 10,000 (left), 1000 (middle), and 100 (right) injected cells (GI-LM2C (red) and GI-LM2G (green)). c Sections of the tumor specimens from experiments in (a) and (b) were stained for hematoxylin and eosin (H&E), Gal3 (IHC), and Gal3 IF (to the left, GI-LM2C, to the right GI-LM2G)