Figure 3.

ADAP is required for dendritic outgrowth. (A) The complexity of dendritic structures is reduced upon ADAP knock down and recovered upon expression of the rescue construct. Dendrite formation was analyzed in primary hippocampal neurons based on the reconstruction of transfected cells via the EGFP signal. Scale bar, 100 μm. (B) Quantification of dendrite branches using Sholl’s method revealed a significant reduction in the total number of dendritic intersections (n = 25–29 per condition). (C) Reduction of intersections is evident in knock-down cells along the entire dendritic arbor, whereas the rescue construct enhances growth over the first 150 μm. Data are Mean ± SEM. *p < 0.01.