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. 2016 Sep 30;9:91. doi: 10.3389/fnmol.2016.00091

Figure 5.

Figure 5

ADAP stimulates MAP2 expression. (A) The immunofluorescence signal of the dendritic marker MAP2 is reduced in ADAP knock-down neurons, compared to control transfected neurons. Scale bar, 100 μm. (B) MAP2 labeling in transfected primary cultures using the In-Cell-Western method confirmed the overall reduction of MAP2 labeling intensity upon ADAP knock down (n = 20–22 wells per condition). Labeling is normalized for the intensity of cell stain, which shows similar density of cells in the different experimental groups. (C) Quantification of In-Cell-Westerns confirms a significant reduction of MAP2 labeling intensity in ADAP knock-down samples. In contrast to dendritic growth measurement, co-expression of the rescue construct does not recover MAP2 expression levels. Data are Mean ± SEM. *p < 0.05.