Figure 6.

ADAP reduces baseline NF-κB activity in hippocampal neurons. (A) Under minimal medium conditions, the NF-κB-induced luciferase signal in primary neurons is reduced in cells with ADAP knock down, irrespective of the presence of the rescue construct. By contrast, when grown under insulin supplementation, a recovery to control levels becomes evident in the rescue group. Application of TNF-α increases luciferase activity in all groups, indicating an efficient activation of the canonical NF-κB pathway (N = 3). (B) mRNA expression of p65 in primary culture peaks at DIV7 (N = 3). Data are Mean ± SEM. *p < 0.05, compared to DIV3.