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. 2016 Jun 10;30(5):393–410. doi: 10.7555/JBR.30.20160040

Fig. 2. Nrf2 upregulation of MGMT expression in MCF-7 and HT29 cells.

Fig. 2

A: Increased MGMT protein levels 24 hours following transient transfection of Nrf2 in MCF-7 cells. The middle panel shows equivalent protein staining on the membrane used for Western blotting. The third panel shows increased DNA repair activity catalyzed by MGMT in Nrf2 transfected cells. B: Increased expression of GST-π and MGMT proteins in HT29 cell clones after stable transfection of Nrf2. Four clones were isolated by G418 selection. The first panel shows the Nrf2 protein expression in these clones and HT29 parent cells. The middle panel displays the enhanced GST-π expression in the clones. The third panel represents the increased expression of MGMT protein in the same background. The bar graph underneath represents the MGMT activity levels in HT29 and its Nrf2 expressing clones. ANOVA revealed that the 2-fold increased DNA repair activity in clones 3 and 4 was significant at P = 0.05. MGMT: O6-methylguanine DNA-methyltransferase; Cont: control; Nrdf2: nuclear factor erythroid 2-related factor.