Fig.3.

Effect of 15B3 on Aβ₄₂ fibrillogenesis, evaluated by ThT fluorescence—Synthetic Aβ₄₂ (4 μM) was incubated with ThT (20 μM) with or without 15B3 or control IgM, and ThT fluorescence was monitored every 2.5 min. Three batches of 15B3 were used for these studies, as indicated. A) Representative raw fluorescent values. B) Normalization of the data in A on the corresponding maximal values to illustrate better the shift in the half-time of transition, i.e., the time corresponding to half the maximum ThT signal. C) Half-time of transition of Aβ₄₂ in the presence of different concentrations of the antibodies. Antibodies are identified by the colors; open or solid symbols indicate results of independent experiments.