Fig.7.

Effect of 15B3 on the ability of Aβ₄₂ oligomers to reduce the pharyngeal motility of C. elegans—Synthetic Aβ₄₂ (100 μM) was incubated at 25°C for 5 h, diluted to 10 μM and incubated with 10 nM or 50 nM 15B3 antibody or 50 nM control IgM, or the vehicle (PBS). The solutions were incubated for another 30 min before being given to the worms. Nematodes were fed for 2 h with these solutions, then plated on Nematode Growth Medium plates seeded with OP50 E. coli. The pharyngeal pumping rate was scored 2 h after plating. Data are expressed as minimum to maximum box and whisker plots (10–20 worms/group from one or two independent experiments). Panels A and B show the results with 15B3 batches # 071114A and # 110531, respectively. ^**p < 0.01 effect of Aβ₄₂ oligomers versus corresponding vehicle; °p < 0.05, °°p < 0.01 effect of 15B3 versus corresponding vehicle, Bonferroni’s test after two-way ANOVA.