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. Author manuscript; available in PMC: 2016 Sep 30.
Published in final edited form as: Cell Rep. 2016 Sep 20;16(12):3348–3358. doi: 10.1016/j.celrep.2016.08.059

Figure 2. The Rrp44da-exosome interface and the CR3/YRD RNA exosome interface are partially redundant.

Figure 2

rrp44-da causes a slow growth phenotype and is synthetic lethal with rrp44-CR3 and rrp44-YRD. An rrp44Δ strain carrying a wild-type RRP44 allele in a URA3 plasmid was transformed with LEU2 plasmids carrying RRP44 variants. Transformants were serially diluted and spotted on 5FOA and SC-LEU-URA (control) media. The western blot indicates that the plasmid-encoded rrp44-da allele is expressed at the same level as endogenous Rrp44 (first lane), or plasmid-encoded wild-type Rrp44 (second lane).