FIG 4.

Effect of dose of virus on the level of latency in TG of latently infected mice. WT C57BL/6 mice were ocularly infected with 1 × 10⁵, 2 × 10⁴, 1 × 10⁴, 2 × 10³, or 1 × 10³ PFU/eye of HSV-1 strain McKrae [LAT⁽⁺⁾] or dLAT2903 [LAT⁽⁻⁾] virus. On day 28 p.i., TG were harvested from the latently infected mice. Quantitative PCR was performed on each individual mouse TG. GAPDH expression was used to normalize the relative expression of viral (gB) DNA in the TG. Each point represents the means ± SEM from 20 TG. The Y-scale for 1 × 10⁴, 2 × 10³, and 1 × 10³ PFU is different from the Y-scale for 2 × 10⁵, 1 × 10⁵, and 2 × 10⁴ PFU. Data for 2 × 10⁵ PFU are repeated from the data shown in Fig. 1. (A) Latency in mice infected with 2 × 10⁵, 1 × 10⁵, or 2 × 10⁴ PFU/eye of LAT⁽⁺⁾ or LAT⁽⁻⁾ virus. (B) Latency in mice infected with 1 × 10⁴, 2 × 10³, or 1 × 10³ PFU/eye of LAT⁽⁺⁾ or LAT⁽⁻⁾ virus.