FIG 3.

Ki67 expression in CD25⁺ FoxP3⁺ and CD25⁻ FoxP3⁻ memory CD4 T cells in HIV⁺ subjects. (A) Representative dot plots for Ki67 staining. (B) Comparison of the frequencies of Ki67⁺ cells in CD25⁺ FoxP3⁺ and CD25⁻ FoxP3⁻ memory CD4 T cells in HIV⁺ subjects. (C) Correlation analysis of the frequency of Ki67⁺ cells between CD25⁺ FoxP3⁺ (y axis) and CD25⁻ FoxP3⁻ (x axis) memory CD4 T cells. (D and E) Correlation analysis of the frequency of Ki67⁺ cells among CD25⁺ FoxP3⁺ (D) and CD25⁻ FoxP3⁻ (E) CD4 T cells versus CD4 T cell frequencies (% of CD3). The analysis was done using cryopreserved PBMC samples from HIV⁺ HHECO study participants. Memory status of CD4 T cells was determined by CD45RA staining. Statistical analysis was performed using the Mann-Whitney test for comparison of groups and the Spearman r statistical test for correlation analyses.