FIG 1.

Analysis of host growth, phage virions, and nucleic acid content throughout the infection cycle of phage ϕ29 in B. subtilis. (A) Phage-mediated lysis of bacterial cultures. Lysis was monitored by measuring the OD₆₀₀ values for samples taken at the indicated times after infection. (B) The ϕ29 virions were counted as PFU per ml of culture. One hundred-fold-diluted cultures were grown in LB medium containing magnesium at 37°C and then infected at an OD₆₀₀ of 0.45 with ϕ29 at a multiplicity of 5. (C) The amount of nucleic acid detected at the indicated times postinfection was quantified by using a 2100 Bioanalyzer (Agilent Technologies), and values are expressed as micrograms of nucleic acid per ml of culture. The graphics are representative of four independent experiments.