FIG 9.
SM111 does not inhibit intracellular p24Gag expression. HEK-293T cells were transfected with wild-type pNL4.3 plasmid, followed by detection of intracellular p24Gag by flow cytometry and extracellular p24Gag by ELISA. (A) Representative flow cytometry data showing intracellular p24Gag expression in the absence or presence of 50 μM or 100 μM SM111. Mean fluorescence intensity (MFI) of p24Gag staining is indicated. (B) Dose-dependent effects of SM111 on intracellular and extracellular p24Gag. (C) Cellular ATP levels for untransfected cells treated with various concentrations of SM111. For panels B and C, means ± SD of results from three independent experiments are shown.