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Cellular Alix is recruited by BFRF1 to modulate the nuclear envelope. Shown are confocal images of HA-BFRF1- and vector-transfected HeLa cells. HA-BFRF1 (red) and emerin or Alix (green) were visualized by indirect immunofluorescence at 3, 6, 9, 12, 18, and 24 h posttransfection. The boxed regions are enlarged on the right.
