TABLE 1.

Oligonucleotides, primers, and plasmid DNA templates used in this study

HA-BFRF1	Deletion or residue substitution	Sequence	Template
ΔESR	Δ(180–313)	5′-ACG CCC AGA AGG CCT CGC GG ACA CCT TAT CTG GCA CGG GT-3′	pcDNA3.0-HA-BFRFl
K49R	K49R	5′-CCC AAC CCC TCT GCA CCG TGA GGC TGC GCC ACG GAC AGA TTT A-3′	pcDNA3.0-HA-BFRFl
K120R	K120R	5′-TCA ACG GAT GTT GAC CTA CCA AGG AAC TCC ATC ATT ATG CTG GGC-3′	pcDNA3.0-HA-BFRFl
K176R	K176R	5′-TTC AGG CCG CCA ACG CCC AGA GGG CCT CGC GGG TCA TGG ATA T-3′	pcDNA3.0-HA-BFRFl
K253R	K253R	5′-GTT ACG CTC CTT GGA GGG ACA GGG ACT CGT GGT CGG AAT CCG A-3′	pcDNA3.0-HA-BFRFl
K265,266R	K265,266R	5′-AAT CCG AGG CGG CGC CGT GGA GGA GGG AAC TCG TGA GGC ACC CCA T-3′	pcDNA3.0-HA-BFRFl
3KR	K120,265,266R	5′-TCA ACG GAT GTT GAC CTA CCA AGG AAC TCC ATC ATT ATG CTG GGC-3′	K265,266R
4KR	K120,176,265,266R	5′-TTC AGG CCG CCA ACG CCC AGA GGG CCT CGC GGG TCA TGG ATA T-3′	3KR
5KR	K120,176,253,265,266R	5′-GTT ACG CTC CTT GGA GGG ACA GGG ACT CGT GGT CGG AAT CCG A-3′	4KR
6KR	K49,120,176,253,265,266R	5′-CCC AAC CCC TCT GCA CCG TGA GGC TGC GCC ACG GAC AGA TTT A-3′	5KR
P75,78A	P75,78A	5′-TAA GCT GAA GAA CTG CAA CTA CGC CTC CTC GGC CGT GTT TGT GAT ATC CAA CAA CG-3′	pcDNA3.0-HA-BFRFl