FIG 2.

Validation of selected CCHFV N protein cellular interaction partners. HEK293T cells were transfected with EGFP–CCHFV-N and EGFP, and, 24 h later, cell lysate components were immunoprecipitated with GFP-trap, RFP-trap, or unconjugated agarose beads. The resulting IPs were separated by PAGE and then subjected to Western blotting to probe for the presence of the selected cellular proteins listed using specific antibodies. The ratios of the abundances of these proteins determined by the two SILAC LC-MS/MS analyses are indicated, and a complete list of all interaction partners is provided as an annotated table in Data set S1 in the supplemental material.