FIG 11.

CD27 expression by cytokine-producing T cells. Intracellular cytokine staining of lymphocytes isolated from blood (PBMC), lungs, and tracheobronchial lymph nodes (TBLN) was performed after in vitro restimulation as described for Fig. 7 to 10. Cytokine-defined subsets identified by Boolean gating were analyzed for CD27 expression. Exemplary contour plots and dot plots show CD27 expression on total (approximately 80,000) CD4⁺ T cells (A) or CD8⁺ T cells (B) and on cytokine-defined subsets. Adjacent bar graphs show the ratio of CD27⁺ (dark gray) and CD27⁻ (light gray) cells within the respective cell population over time. Each column within a time frame corresponds to an individual animal. The red line connects median values for CD27⁺ cells of 5 animals per time point. The dotted horizontal line represents the 50% mark. For more accurate representation of subsets, the dot plot showing singly IFN-γ-producing CD8⁺ T cells in the lung (#) was set to display only 10% of events.