FIG 8.

Production of IFN-γ, TNF-α, and IL-2 by CD8⁺ T cells. Intracellular cytokine staining was performed following overnight in vitro restimulation of freshly isolated cells with FLUAVsw (infection strain, MOI = 0.1; 18 h). Contour plots show cytokine production of CD8⁺ T cells (gated on CD4⁻ CD8β⁺ cells [not shown]) isolated from blood (PBMC), lungs, tracheobronchial lymph nodes (TBLN), and mesenteric lymph nodes (MesLN) of animals representative for each time point postinfection. Approximately 120,000 CD8⁺ T cells are shown in each contour plot.