FIG 3.

No apparent role of the CDP-choline pathway in HCV replication. (A) Huh7 cells remained untransfected or were transfected with the indicated siRNAs and then mock infected or infected with HCV. Cell lysates were subjected to immunoblot analysis. (B and C) Huh7 cells were mock infected or infected with HCV and then treated with different concentrations of CK37 or HePC, as indicated. Cells lysates were analyzed by immunoblotting. (D) Huh7 cells remained mock infected or were inoculated with untreated or UV-irradiated HCV. (E) Huh7 cells were first transfected with the indicated siRNAs and then mock infected or infected with HCV. (F) Huh7 cells were mock infected or infected with HCV and then treated with DMSO (−), CK37, HePC, or AICAR, as indicated, for 24 h. For the experiments shown in panels D to E, a set of 10⁶ cells was used to determine the PC amount (top panel), while another set was analyzed by Western blotting (bottom panel). (G) The viability of Huh7 cells treated with vehicle or the indicated inhibitors was assessed. (H) The PC levels in control and hCKα stable knockdown cells were measured. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, nonsignificant.