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. 2016 Sep 29;90(20):9075–9095. doi: 10.1128/JVI.00960-16

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Copyright © 2016 Wong and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — HCV-induced increase in the distribution of hCKα into ER membranes by HCV infection. (A) The postnuclear fractions from mock- and HCV-infected Huh7 cells were subjected to iodixanol-based OptiPrep density gradient ultracentrifugation. Proteins in each fraction were precipitated with 10% cold trichloroacetic acid and then analyzed by Western blotting for the indicated proteins. Extracts from mock- and HCV-infected cell lysates were separately loaded as controls for the detection of the indicated proteins (P lanes). (B) The intensity of hCKα in each fraction was quantified by ImageQuant, and the percentage of hCKα fractionated into LDs (fractions 1 to 7) and the ER (fractions 8 to 14) over the total hCKα level obtained from mock- and HCV-infected cells was quantified. ****, P < 0.0001.