FIG 9.

The effect of hCKα knockdown on ER localization of NS5A, NS5B, and NS3. The control and hCKα shRNA Huh7 stable cells were transfected with pTM-NS3-5B and pWPI-T7-BLR, and the cells were examined by confocal microscopy for ER localization of NS5A (A), NS5B (B), and NS3 (C) (left panels). The white boxed areas in the NS/CALR images were enlarged (Zoom), and white arrowheads indicate the ER localization of each indicated NS protein. The extent of colocalization of each NS protein with CALR in control and hCKα shRNA cells was quantified (right panel). ****, P < 0.0001; ns, nonsignificant.