FIG 1.

Protein synthesis by NS segment reassortant viruses in mammalian cell culture. MDCK cells were infected at an MOI of 3, and cell lysates were prepared at 8 h p.i. To detect viral NP, NS1, M1, and M2 polypeptides as well as cellular tubulin, lysates were subjected to SDS-PAGE and immunoblotted with the appropriate antisera. To detect NEP, cells were infected at an MOI of 10 and metabolically labeled with a ³⁵S protein labeling mix between 6 h and 8 h p.i. The lysates were then immunoprecipitated with anti-NEP antiserum, and precipitates were analyzed by SDS-PAGE and autoradiography. Data are representative of those from more than one independent experiment.