FIG 9.

Role of TLR2 in viral replication and expression of IL-6 and CXCL1 in MHV3-, 51.6-MHV3-, and YAC-MHV3-infected LSECs. (A) LSECs were infected at an MOI of 0.1 with MHV3, 51.6-MHV3, and YAC-MHV3. At 24, 48, and 72 h p.i., RNA from infected LSECs was extracted and mRNA expression levels for the TLR2 gene were determined by qRT-PCR. (B to D) LSECs were treated with a specific siRNA against TLR2 prior to infection with viruses or treatment with the specific TLR2 agonist Pam3Cys (as a positive control) for 24 h. mRNA expression levels of MHV nucleoprotein (MHV-N) (B), IL-6 (C), and CXCL1 (D) were determined by qRT-PCR. Values represent fold change in gene expression relative to that in control (uninfected) LSECs after normalization to HPRT expression. All samples were run in duplicate, and results are representative of two independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (compared with control cells). †, P < 0.05 (compared with MHV3-infected cells).