FIG. 1.

(A–F) Inverted microscopic images of the scratch “wound” assay at 100× magnification. The black line outlines the wound margin with cells located on the edges and the “mock” wound devoid of cells. (A) Captured at 0 hours, immediately after the wound was created. (B) Captured at 4 hours postwound; the cells have begun to migrate to cover the wound site. (C) Captured at 8 hours postwound; the cells continue to migrate to cover the wound site. (D) Captured at 12 hours postwound; the cells continue to migrate to cover the wound site. (E) Captured at 16 hours postwound; the cells have completely invaded the wound site, indicating 100% wound closure. (F) Captured at 20 hours postwound. All scale bars = 200 μm.