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. Author manuscript; available in PMC: 2016 Sep 30.
Published in final edited form as: Sci Transl Med. 2015 May 20;7(288):288ra80. doi: 10.1126/scitranslmed.aaa4097

Figure 6. High-dose acetaminophen exposure and Leydig cell number in the rat fetal testis.

Figure 6

Treatments commenced at e13.5 and tissue was collected at e17.5, 3 h after the final treatment. A) Double immunofluorescence for 3β-HSD (Leydig cells; green) and Sox-9 (Sertoli cells; red) in vehicle- (VEH; A) and acetaminophen- (ACET; B) exposed rat fetal testes. Scale bar – 50 μm. C) Quantification of Leydig cell to Sertoli cell ratio (LC:SC) and D) Leydig cells/mm2 for acetaminophen (ACET; red) or vehicle (VEH; blue) exposure (n=4). Graphs show mean +/- SEM. Data analyzed by unpaired t-test.