Figure 3.

Effects of hypoxia on low-density lipoprotein-induced cytotoxicity.

Notes: THP-1-derived macrophages were incubated for 72 hours with 200 μg/mL of low-density lipoproteins under hypoxia (1% oxygen) or normoxia (21% oxygen). After incubation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was added to the medium and the cells were placed for 2 hours and 30 minutes in the dark at 37°C in the presence of 5% carbon dioxide. Cells were then lysed and optical density was measured at 570 nm. Results are expressed as the mean of three independent experiments ± standard deviation. ***P<0.001 versus time-matched normoxic control. **P<0.01; ***P<0.001.

Abbreviations: CM, cell-modified/native low-density lipoproteins; CTL, control; H, hypoxia; Mox, myeloperoxidase-modified low-density lipoproteins; N, normoxia; Ox, copper sulfate-oxidized low-density lipoproteins.