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. 2015 Sep 18;3:35–43. doi: 10.2147/HP.S92198

Figure 2.

Figure 2

O2 concentration gradients in cell culture medium.

Notes: Normal 24-well tissue culture plates without (left) or with (right) confluent HeLa cell layers were removed from a normoxic 37°C incubator, and O2 concentration profiles were determined under room air conditions at 25°C using a needle-type O2 sensor (PreSens, Regensburg, Germany). Note that the change from incubator air to room air results in a higher pO2 (no gaseous water, no pCO2) and a better O2 solubility (temperature change from 37°C to 25°C).