Figure 1.

Oxygen-dependent regulation of the lncRNAs NEAT1 and MALAT1 in vitro.

Notes: The indicated cell lines were exposed to 20% (normoxia) or 0.2% (hypoxia) O₂ for 24 hours. lncRNA levels were determined by RT-qPCR and displayed as ratio to the mRNA levels of the housekeeping ribosomal protein L28 which served as constitutive control. Shown are mean values + SEM of three independent experiments. The overall significance of the hypoxic induction of both lncRNAs was P<0.05 as assessed by Student’s t-test.

Abbreviations: lncRNAs, long noncoding RNAs; NEAT1, nuclear-enriched abundant/autosomal transcript 1; MALAT1, metastasis-associated lung adenocarcinoma transcript 1; SEM, standard error of mean; RT-qPCR, quantitative reverse transcription polymerase chain reaction.