Figure 4.

In situ expression of the lncRNA MALAT1 in vivo. Mice were exposed to normoxia or hypoxia (8% O₂) for 24 hours.

Notes: In situ hybridization was performed with digoxigenin-labeled antisense or sense probes followed by detection with alkaline phosphatase-labeled anti-digoxigenin antibodies and nitro-blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate staining using kidney (A) and testis (C) tissue slices. (B) MALAT1 positive renal proximal and distal tubules of three visual fields were counted and shown as mean values + SEM of three independent sections (*P<0.001, Student’s t-test).

Abbreviations: h, hours; lncRNAs, long noncoding RNAs; MALAT1, metastasis-associated lung adenocarcinoma transcript 1; SEM, standard error of mean.