Figure 5.

Oxygen-dependent regulation of the lncRNAs NEAT1 and MALAT1 in primary renal epithelial cells.

Notes: Epithelial cells were isolated from mouse kidney and exposed to 20% (normoxia) or 0.2% (hypoxia) O₂ for 24 hours. β-Actin mRNA and lncRNA levels were determined by RT-qPCR and corrected for the mRNA levels of the housekeeping ribosomal protein S12 which served as internal control. Shown are hypoxic induction factors as mean values + SEM of three independent experiments (*P<0.05, Student’s one-tailed t-test).

Abbreviations: lncRNAs, long noncoding RNAs; mRNA, messenger RNA; NEAT1, nuclear-enriched abundant/autosomal transcript 1; MALAT1, metastasis-associated lung adenocarcinoma transcript 1; RT-qPCR, quantitative reverse transcription polymerase chain reaction; SEM, standard error of mean.