Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 18;7(1):243–254. doi: 10.4161/19420862.2014.985132

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

PMC Copyright notice

Figure 4. — Correlation between affinity and potency of toxin cross-neutralizing mAbs. (A): Neutralization potency of toxin cross-reactive mAbs Hla-F#1-7 was determined with rabbit RBCs for Hla, and with freshly isolated human PMNs for HlgCB, LukED and LukSF and expressed as mAb:toxin ratio at half maximal inhibition of cell lysis (IC₅₀). For all toxins the correlation between in vitro potency and affinity was significant based on Pearson's correlation coefficient analysis (r > 0.9, P < 0.005). In vivo potency of mAbs was determined in intravenous challenge of mice with HlgAB (B) and HlgA-LukD (C) or with intranasal challenge with Hla (D). Groups of 5 mice were given 100 μg of indicated mAbs intraperitoneally before challenge with 100% lethal dose of toxins. Fab affinities (K_D) measured by MSD are indicated.