Figure 5.

Inhibition of native cytolysins with toxin cross-reactive and Hla-specific mAbs. Target cells were intoxicated with sterile filtered bacterial culture supernatants (CS) after pre-incubation with Hla-F#5 cross-reactive and indicated Hla-specific mAbs. (A): Human lung epithelial cells (A549) with CS from TCH1516 strain; (B): human RBCs with CS from Newman strain; (C): human PMNs with CS from TCH1516ΔlukGH strain. (D–G): rabbit RBCs with CS from TCH1516 strain at 17.5×, 7.5× and 2.5× dilutions (D, E and F, respectively) and with CS from the TCH1516ΔhlgABCΔlukEDΔlukSFΔlukGH strain at 4× dilution. Inhibition of supernatant cytotoxicity by mAbs was measured by determining cell viability or hemolysis in case of RBCs. Error bars represent SD, n = 3.