Table 1.

Binding affinity (Fab K_D) measured by Meso Scale Discovery (MSD) and in vitro neutralization potency of mAbs used in the study

					In vitro neutralization potency
	Affinity (MSD Fab KD, pM)				IC50 expressed as mAb: toxin ratio
	Hla	HlgB	LukF	LukD	Hla	Hla	HlgCB	HlgAB	LukSF	LukED	HlgA-LukD	Hla LukSF HlgCB HlgAB LukED	Hla LukSF HlgCB HlgAB LukED
mAbs					rRBC	A549	hPMN	hRBC	hPMN	hPMN	hRBC	hPMN	hRBC
Hla-F#1	2.9a	5.1	120	2200	<0.25	<0.5	1.2	13.5	7.8	4.9	8.2	3.5	2.9
Hla-F#2	37a	18	< 3	1700	<0.25	0.6	0.7	6.5	0.5	4.8	7.3	1.1	1.6
Hla-F#3	23a	140	nd	86	<0.25	<0.5	147.6	nd	nd	0.3	1.2	nd	nd
Hla-F#4	30a	160	nd	160	<0.25	<0.5	180.7	nd	nd	0.4	1.4	nd	nd
Hla-F#5	<2a, 2.1b	< 3	< 3	400	< 0.25	< 0.5	0.7	5.6	0.7	1.0	3.0	0.5	1.1
Hla-F#6	< 2a	< 2	50	290	< 0.25	< 0.5	0.8	2.8	2.2	0.9	2.3	0.8	0.6
Hla-F#7	< 2a	< 2	73	780	< 0.25	< 0.5	0.3	4.2	2.7	2.2	4.8	1.2	0.9
Hla	40b	nd	nd	nd	< 0.25	< 0.5	nd	nd	nd	nd	nd	nd	nd
HlgB	nd	4.2	nd	nd	nd	nd	1.6	1.6	nd	nd	nd	nd	nd
LukD	nd	nd	nd	7.0	nd	nd	nd	nd	nd	0.2	1.2	nd	nd
LukF	nd	nd	9000	nd	nd	nd	nd	nd	93.4	nd	nd	nd	nd
243-4	13b	nd	nd	nd	< 0.25	< 0.5	nd	nd	nd	nd	nd	nd	nd
LC10	13b	nd	nd	nd	< 0.25	< 0.5	nd	nd	nd	nd	nd	nd	nd
LTM14	4.5b	nd	nd	nd	< 0.25	< 0.5	nd	nd	nd	nd	nd	nd	nd

nd: not detectable

^a K_D values of yeast produced mAbs measured in the same assay (fitted antigen concentrations between 20 and 30 nM were used to calculate lower K_D limits (10% of the antigen concentration); ^b K_D values of mammalian cell produced mAbs measured in the same assay at 20 pM antigen concentration (K_D limit ∼ 2 pM). 95% confidence intervals of the predicted K_Ds are typically within two fold or less from the fitted K_D values reported here.