Figure 5.

Humanized variants of TZ47. Initially 12 designs with 25 mutations were generated (A) and 4 diverse designs were selected according to a tree-based representation of sequence identity (B). Humanized variants are named according to the combination of (design # VH) / (design # VL). There are 2 unique light chains and 4 heavy chains; their possible combinations were also considered (A). Design 4 has 7 light-chain and 18 heavy-chain mutations, whereas Design 10 and 11 have 8 light and 17 heavy mutations. Thus the combinations 4H/10L and 9H/10L have 26 mutations in total (24 mutations for 11H/4L and 10H/4L) and are not on the Pareto optimal curve of designs with 25 mutations.