Fig 3. eif-3.K mutants do not have attenuated bulk translation.

(A) Polysome profiles of the indicated genotypes, harvested at the L4 stage. Identical quantities of total RNA were loaded onto sucrose gradients, and absorbance was measured at 254 nm. (B) Relative translation assayed by ribosome profiling, using whole yeast lysates as an internal standard. Whole lysates from S. cerevisiae were added to whole C. elegans lysates at a ratio of 1:20 based on total RNA concentration. Following ribosome profiling, the relative abundance of ribosome protected fragments (RPF) was determined by dividing the total number of reads that map unambiguously to the C. elegans genome by those that map unambiguously to the S. cerevisiae genome. Raw counts are presented in S1 Table. Statistical significance was assessed by the Student’s t-test.