Fig 1. Insulin-producing cells (IPCs) regulate reproductive dormancy in D. melanogaster.

(A) Ablation of IPCs with dilp2>hid,rpr and Insp3>hid,rpr significantly enhances diapause at both 11 and 28 days compared with controls even under a long summer photoperiod (LD16:8). (B) Hyperexcitability of IPCs decreases diapause frequency (NaChBac) whereas reducing excitability (Ork1) significantly enhances diapause also compared to the non-conducting control NOrk1. Blue 11 days, red 28 days. Numbers within bars represent replicates (see Methods). Mean ± SD, ***p<0.001. (ANOVA was performed using the arcsin transformation but the results are presented as percentages for simplicity).