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. 2016 Sep 30;11(9):e0163680. doi: 10.1371/journal.pone.0163680

Fig 5. Regulation of FoxO and dilp2/5 in diapausing females.

Fig 5

(A) FoxO.RE-Luciferase reporter gene assay. Reporter activity in diapausing (12°C) versus non-diapausing flies (23°C) is shown (p = 0.032, t-test). Reporter activity is higher in flies abdomen at 12°C. Y-axis: Luciferase activity (fold change). (B) qPCR of dilps-2/5 mRNA levels in two different ‘wild-type’ genotypes, FoxO.RE.Luciferase used in A and dilp2>+. dilps-2/5 expression levels of diapausing (12°C) versus non-diapausing flies (23°C) are shown (ratio: diapausing/non-diapausing). dilps-2/5 are up-regulated in diapausing flies. Y-axis: mRNA levels (fold change). Dotted line indicates the expression levels in non-diapausing flies. Mean ± SE, *p<0.05, ***p<0.001 are based on t-test.