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. 2016 Sep 30;11(9):e0163751. doi: 10.1371/journal.pone.0163751

Fig 3. GM-CSF, LPS and Siglec-F signaling enhance IL-33-induced secretion of IL-4 and IL-13 from eosinophils.

Fig 3

(A) BMDE were cultured with IL-5 and indicated concentrations of IL-33 and LPS. (B) BMDE were cultured in 10 ng/ml IL-5 and IL-33 in the presence or absence of 10 ng/ml GM-CSF. In addition, anti-Siglec-F, isotype control antibody or no antibodies were added to the culture. (C) BMDE were cultured in 10 ng/ml IL-5 and IL-33. In addition, the p38 kinase was blocked with 5 μM SB203580 and DMSO was used as solvent control. All supernatants were analyzed at 24 hours after onset of culture by IL-4 and IL-13 specific ELISAs. Bars show the mean + SD from one of two independent experiments with similar results (A; n = 4) the mean + SD from one of three independent experiments (B; n = 4) and mean + SEM pooled from two independent experiments (C; n = 8) *p<0.05; **p<0.01; *** p<0.001).