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. 2016 Sep 30;11(9):e0163751. doi: 10.1371/journal.pone.0163751

Fig 4. IL-33 activated eosinophils mediate alternative activation of macrophages.

Fig 4

(A) and (B) BMDM generated from wild-type BALB/c mice were co-cultured with BMDE from either wild-type (black bars) or IL-4/IL-13-deficient BALB/c mice (grey bars) for 24 hours in the presence of the indicated cytokines. Quantitative RT- PCR was performed to analyze Relm-α and Arginase-1 expression as established markers for alternative activation of macrophages. Data are presented as normalized expression to PBGD. Bars show the mean + SD (n = 3) from one of three independent experiments with similar results. (C) and (D) only supernatants of IL-5+IL-33+GM-CSF stimulated BMDE from WT (black bars) or IL-4/IL-13-deficient mice (grey bars) were added to BMDM for 24 hours before quantitative RT-PCR was performed. Bars show the mean + SD (n = 3) from one experiment.